Olympus TIRF
Imaging configuration: Widefield
Microscope stand: Olympus IX81, Inverted
TIRF illumination system: Olympus
Dual channel detection system: Andor
Fluorescence excitation: Olympus CW lasers
LASER LINE (NM) | MAX OUTPUT POWER (mW) |
405 | 100 |
488 | 150 |
561 | 150 |
640 | 100 |
Detection: 2 channels
Detection devices: 2x EM-CCD, Andor iXon X3 DU897
Chip size: 512x512 pixels
Pixel size: 16x16 µm
Dynamic range: 14 bit
Maximum frame rate: 35 frames/s (full chip)
Quantum efficiency: > 90% @ 600 nm
Control and acquisition software: Olympus cell^TIRF and Andor IQ3
Additional features:
XY motorized stage
XYZ piezo stage (+- 100 µm)
Z drift compensation module Olympus ZDC2
Incubator box from OKO Lab for temperature control
Objectives (Olympus)
TYPE | MAGNIFICATION | NUMERICAL APERTURE | MEDIUM | CONTRAST | WORKING DISTANCE (MM) |
ApoNTIRF | 60x | 1.49 | Oil | Fluo, DIC | 0.1 |
UPlanFLN | 100x | 1.3 | Oil | Fluo, DIC, phase | 0.2 |
UApoNTIRF | 150x | 1.45 | Oil | Fluo, DIC | 0.08 |
Dichroic mirrors for TIRF illumination installed in the microscope
LASER LINES REFLECTED | REFERENCE |
405/488/561/640 | Di01-R405/488/561/635 |
488/561 | Di01-R488/561 |
Detection splitting filter cubes for dual channel detection
Dichroic filter | Emission filter ch1 | Emission filter ch2 | Filter reference |
552 | 524/29 | 630/92 | FF552-Di02, FF01-630/92, FF01-630/92 |
580 | 537/26 | 630/69 | FF580-FDi01, FF01-537/26, FF01-630/69 |
662 | 617/73 | OPEN | FF662-FDi01, FF01-617/73 |
Filter cubes installed in the microscope for conventional epi-fluorescence
Fluorophore | Excitation filter | Dichroic filter | Emission filter | Filter cube reference |
GFP | 482/18 | 495 | 520/28 | GFP-1828A-OMF-ZERO |
mCherry | 562/40 | 593 | 641/75 | mCherry-B-OMF-ZERO |
Cy5 | 628/40 | 660 | 692/40 | Cy5-4040B-OMF-ZERO |
