Nikon confocal A1R/Sim
Imaging configuration: laser scanning / confocal
structured illumination / widefield (SIM)
Microscope stand: Nikon Eclipse Ti, inverted
Laser scanning confocal microscope:
Fluorescence excitation: CW lasers
Laser line (nm) | Max output power (mW) | Ref |
405 | 100 | Coherent Cube |
457 | Melles-Griot IMA 1010 40 ALS | |
477 | Melles-Griot IMA 1010 40 ALS | |
488 | 16 | Melles-Griot IMA 1010 40 ALS |
514 | 13 | Melles-Griot IMA 1010 40 ALS |
561 | 20 | Coherent Sapphire |
640 | 40 | Coherent Cube |
Detection: 4 channels
Detection devices: 2x conventional PMTs (10-20% quantum efficiency)
2x GaAsP (40% quantum efficiency between 450-600 nm)
Control and acquisition software: Nikon NIS
Additional features:
XY mechanical stage
Z piezo stage (+- 100 µm)
Z drift compensation module Nikon Perfect Focus System (PFS)
Galvano scanner mirrors
Resonant scanner mirrors
Transmitted image acquisition
Incubator chamber from Tokai Hit for temperature and Air/CO2 mixing control
Objectives (from Nikon)
Type | Magnification | Numerical Aperture (NA) | Medium | Contrast | Working Distance (mm) |
Plan Apo λ | 10x | 0.45 | Air | Fluo, DIC | 4 |
Plan Apo λ | 20x | 0.75 | Air | Fluo, DIC | 1 |
Plan Apo IR | 60x | 1.27 | water | Fluo, DIC, Phase | 0.17 |
SR Apo TIRF | 100x | 1.49 | oil | Fluo, DIC, Phase | 0.12 |
Excitation dichroics
- 405/488
- 405/488/561
- 405/488/561/640
- 400-457/514
- 405/488/543/640/
- Beam splitter 20/80
Channel 1: conventional PMT detector
Dichroic beam-splitter | fluorescence emission filter |
495 LP | 450/50 |
515LP | 482/35 |
Channel 2: GaAsP detector
Dichroic beam-splitter | fluorescence emission filter |
560 LP | 525/50 |
640LP | 540/30 |
Channel 3: GaAsP detector
Dichroic beam-splitter | fluorescence emission filter |
640LP | 595/50 |
Channel 4: conventional PMT detector
Dichroic beam-splitter | fluorescence emission filter |
| 700/75 |
Structured illumination microscope (SIM):
Fluorescence excitation: CW lasers
Laser line (nm) | Max output power (mW) | Ref |
457 |
| Coherent |
488 |
| Coherent |
515 |
| Coherent |
561 |
| Coherent |
640 |
|
|
Detection: 1 channel
Detection devices: EM-CCD Andor iXon X3 DU897
Chip size: 512x512 pixels
Pixel size: 16x16 µm
Dynamic range: 14 bit
Maximum frame rate: 35 frames/s (full chip)
Quantum efficiency: > 90% @ 600 nm
Control and acquisition software: Nikon NIS
Additional features:
XY mechanical stage
Z piezo stage (+- 100 µm)
Z drift compensation module Nikon Perfect Focus System (PFS)
2D SIM / 3D SIM / widefield
TIRF SIM
Incubator chamber from Tokai Hit for temperature and Air/CO2 mixing control
Objectives (from Nikon)
Type | Magnification | Numerical Aperture (NA) | Medium | Contrast | Working Distance (mm) |
SR Apo TIRF | 100x | 1.49 | Oil | Fluo, DIC | 0.12 |
