Andor Confocal Spinning Disk
Imaging configuration: Confocal spinning disc / widefield
Microscope stand: Olympus IX81, Inverted
Illumination and detection system: Andor Revolution and Yokogawa CSU X1
Fluorescence excitation: CW lasers, Andor Revolution
Laser Line (nm) | Max output power (mW) |
405 | 100 |
445 | 40 |
491 | 100 |
515 | 50 |
561 | 50 |
640 | 100 |
Detection: 1 channel
Detection devices: EM-CCD Andor iXon X3 DU897
Chip size: 512x512 pixels
Pixel size: 16x16 µm
Dynamic range: 14 bit
Maximum frame rate: 35 frames/s (full chip)
Quantum efficiency: > 90% @ 600 nm
Control and acquisition software: Andor IQ3
Additional features:
FRAPPA unit for FRAP experiment
XY motorized stage
Z piezo stage (+- 100 µm)
Z drift compensation module Olympus ZDC2
Incubator box from OKO Lab for temperature control
Objectives (Olympus)
Type | Magnification | NumericalAperture (NA) | Medium | Contrast | Working distance (nm) |
PlanN | 20x | 0.4 | Air | Fluo | 1.2 |
UPlanFLN | 60x | 1.25 - 0.65 | Oil | Fluo, DIC | 0.12 |
UPlanSApo | 100x | 1.4 | Oil | Fluo, DIC | 0.13 |
Dichroic mirror for Spinning disc illumination
Laser Lines transmitted | Reference |
405/488/561/640 | Di01-T405/488/568/647 |
others to be determined |
Fluorescence emission filters
Emission filter | Type | Reference |
542/27 | Band-pass | FF01-542/27 |
617/73 | Band-pass | FF01-617/73 |
685/40 | Band-pass | FF01-685/40 |
690LP | Long-pass | HQ690LP |
447/55 | Band-pass | FF01-447/55 |
475/42 | Band-pass | FF01-475/42 |
525/50 | Band-pass | FF01-525/50 |
Filter cubes for installed in the microscope for conventional epi-fluorescence
Fluorophore | Excitation filter | Dichroic filter | Emission filter | Filter cube reference |
DAPI | 387/11 | 409 | 447/60 | DAPI-1160B-OMF-ZERO |
CFP | 438/24 | 458 | 483/32 | CFP-2432B-OMF-ZERO |
GFP | 482/18 | 495 | 520/28 | GFP-1828A-OMF-ZERO |
YFP | 500/24 | 520 | 542/27 | YFP-2427B-OMF-ZERO |
Cy5 | 628/40 | 660 | 692/40 | Cy5-4040B-OMF-ZERO |
