Elizabeth was awarded a small grant (Eur 30.000) in the ZonMW Enabling Technologies Hotels program.
The project “Correlative Light and Electron Microscopy to Assess Synapse Development in Zebrafish Larvae” is in collaboration with the Koster group, LUMC, and Delmic.
Our goal is to develop sample preparation workflow to use live light imaging followed by correlative light-electron microscropy to investigate how synapses form in individual neurons in the zebrafish brain.
The above figure is an annotated large scale electron microscopy image of a 5 day old zebrafish, made by the Koster lab at LUMC: F. G. Faas, C. M. Avramut, B. M. van den Berg, M. A. Mommaas, A. J. Koster, and R. B. Ravelli, “Virtual nanoscopy: generation of ultra-large high resolution electron microscopy maps,” The journal of cell biology, vol. 198, iss. 3, pp. 457-469, 2012.
Our project together will fluorescently label small numbers of cells so we can track them in life and find the same cells again in the electron micrograph.