Olympus widefield and Confocal rescan
Purpose:
- Suitable for widefield fluorescence and transmission imaging
- Instant shift to confocal rescan imaging (slow). Pinhole size= 50 µm, Lateral resolution: 170 nm (FWHM at 488 nm excitation); Axial resolution: 500 nm (at 488 nm excitation); excitation: 405 - 488 - 561 - 638 nm
- Dedicated to short-term experiments, up to 4h
Illumination / Imaging configuration: Widefield / Confocal
Imaging contrasts: Brightfield, and Fluorescence
Microscope stand: Inverted Olympus IX81, motorized
Fluorescence excitation source WF: CoolLED pE-4000, LED light source
Fluorescence excitation confocal: Toptica iChrome CLE lasers @20mW: 640nm; 561nm; 488nm; 405nm
Confocal band pass filters, MOUNTED ON Infinity optics filterwheel
(S1050/001/INF Cairn 1x C‐mount infinity port ):
ET450/50
ET525/36
ET600/50
ET655lp
Detection: 1 channel
Detection device: ORCA-Flash 4.0
| Product number | C133440-20CU |
| Imaging device | sCMOS |
| Cell (pixel) Size (µm2) | 6.5x6.5 |
| Pixel array (horizontal by vertical) | 2048x2048 |
| Effective area (horizontal by vertical in mm) | 13.312x13.312 |
| Peak Quantum Efficiency (QE) | 82% @560 nm |
| Dynamic range | 37000 : 1 |
Acquisition software: INSCOPER: AUTOMATION & IMAGE ACQUISITION SOLUTION for Fluorescence Microscopy
Additional features:
- XY motorized stage
- Hardware Z-drift compensation (Olympus ZDC2)
Objectives:
| Objective type | Mag/NA | Medium | Contrast | WB (mm) | Z-Drift compensation system | Pixel size (nm) |
| UPlanFLN | 20x / 0.5 | Air | Fluo, Ph | 2.1 | / | 397 |
| PlanN | 40x / 0.65 | Air | Fluo | 0.6 | / | 200 |
| PlanApoN | 60x / 1.42 | Oil | Fluo | 0.15 | YES | 133 |
| UplanSApo | 100x / 1.4 | Oil | Fluo | 0.13 | YES | 79 |
Fluorescence filter cubes installed:
Fluorophore | Excitation filter | Dichroic filter cut-off | Emission filter | Filter cube reference |
DAPI | 387/11 | 409 | 447/60 | DAPI-1160B |
GFP | 482/18 | 495 | 520/28 | GFP-1828A |
YFP | 500/24 | 520 | 542/27 | YFP-2427B |
mCherry | 562/40 | 593 | 593LP | mCherry-40LP-A |
Cy5 | 628/40 | 660 | 692/40 | Cy5-4040B |
