Nikon confocal A1R / SIM Scanning confocal

Purpose:
- High resolution 3D confocal sectioning
- Live experiments with Resonant scanner and Denoise.ai function
- Smart acquisition using Nikon NIS.ai 
- FRAP
- Structured illumination: 2D SIM / 3D SIM 

Microscope stand: Nikon Eclipse Ti, inverted, equipped with full environmental control
Fluorescence excitation:
1. Confocal-CW lasers: 405nm@100 mW ; 457nm ; 477nm ; 488nm@16mW ; 514nm@ 13mW ;561nm@20mW; 640nm@ 40mW 
2. SIM-CW lasers: 457nm;  488nm;  515nm;  561nm;  640nm  

Confocal detection: 4 channels
Detection devices: 2x conventional PMTs (10-20% quantum efficiency), 2x GaAsP (40% quantum efficiency between 450-600 nm)

SIM detection: 1 channel
Detection device: EM-CCD Andor iXon X3 DU897
Chip size: 512x512 pixels
Pixel size: 16x16 µm
Dynamic range: 14 bit
Maximum frame rate: 35 frames/s (full chip)
Quantum efficiency: > 90% @ 600 nm

Control and acquisition software: Nikon NIS
Additional features:
- XY mechanical stage
- Z piezo stage (+- 100 µm)
- Z drift compensation module Nikon Perfect Focus System (PFS)
- Galvano scanner mirrors
- Resonant scanner mirrors
- Transmitted image acquisition
- Incubator chamber from Tokai Hit for temperature and Air/CO2 mixing control

Objectives (from Nikon):

Excitation dichroics:
- 405/488
- 405/488/561
- 405/488/561/640
- 400-457/514
- 405/488/543/640/
- Beam splitter 20/80

Fluorescence filters: