CellInsight CX7 High Content
*E1.310 is ML-1 mammalian cell culture room, kept sterile. Proper training should be given before gaining access to the room. Responsible person for cell culture: Cátia Pereira Frias.
Purpose:
High-throughput automated confocal imaging with built in analysis platform. Dedicated to live/fixed mammalian cells.
Microscope stand: CellInsight CX7
Confocal: high-speed CREST spinning disc with 40&70µm pinholes
Illumination: solid state, 7 color LED excitation + white light for brightfield contrast. Software control over intensity.
Fluorescence excitation:
Picoquant picosecond pulsed lasers
- 386/440 nm
- 438/480 nm
- 485/521 nm
- 549/600 nm
- 560/607 nm
- 650/694 nm
- 747/810 nm
Detection: Photometrics™ X1 CCD camera
High-sensitivity, thermoelectrically cooled to 0°C
14-bit data acquisition and processing
Field of view (FOV): 2,208 x 2,208 pixel array
4.54 μm pixel size
75% peak quantum efficiency (QE)
Objectives:
Objective type | Mag/NA | Medium | Contrast | WB (mm) |
UPLFLN | 10x/0.3 | Air | Flou,DIC | 10 |
LUCPLFLN | 20X/0.45 | Air | Fluo, DIC | 6.6-7.8 |
UCPLFLN | 20X/0.7 | Air | Fluo, DIC | 0.8-1.8 |
LPLN | 40X/0.6 | Air | Fluo | 3-4.1 |
UPLFLN | 60X/0.9 | Air | Fluo, DIC | 0.2 |