CellInsight CX7 High Content


*E1.310 is ML-1 mammalian cell culture room, kept sterile. Proper training should be given before gaining access to the room. Responsible person for cell culture: Cátia Pereira Frias. 

Purpose:
High-throughput automated confocal imaging  with built in analysis platform. Dedicated to live/fixed mammalian cells. 

Microscope stand: CellInsight CX7
Confocal: high-speed CREST spinning disc with 40&70µm pinholes
Illumination: solid state, 7 color LED excitation + white light for brightfield contrast. Software control over intensity.
Fluorescence excitation:  
Picoquant picosecond pulsed lasers
- 386/440 nm
- 438/480 nm
- 485/521 nm
- 549/600 nm
- 560/607 nm
- 650/694 nm
- 747/810 nm

Detection: Photometrics™ X1 CCD camera

High-sensitivity, thermoelectrically cooled to 0°C
14-bit data acquisition and processing
Field of view (FOV): 2,208 x 2,208 pixel array
4.54 μm pixel size
75% peak quantum efficiency (QE)

Objectives:

Objective type Mag/NA Medium Contrast WB (mm)

UPLFLN

10x/0.3

Air

Flou,DIC

10

LUCPLFLN

20X/0.45

Air

Fluo, DIC

6.6-7.8

UCPLFLN

20X/0.7

Air

Fluo, DIC

0.8-1.8

LPLN

40X/0.6

Air

Fluo

3-4.1

UPLFLN

60X/0.9

Air

Fluo, DIC

0.2