Genome engineering and molecular toolbox

Related publications

Swiat, M., S. Dashko, M. Den Ridder, M. Wijsman, J. van der Oost, J.M. Daran, P. Daran-Lapujade. 2017. FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae. Nucleic Acids Res. In press. Doi:10.1093/nar/gkx1007

Kuijpers, N.G.A., D. Solis-Escalante, M.A.H. Luttik, M. van den Broek, J.T. Pronk, J.-M. Daran, and P. Daran-Lapujade. 2016. Pathway swapping: towards modular engineering of essential cellular processes. Proc.Natl.Acad.Sci.USA. 113:15060

Mans, R., H. M. van Rossum, M. Wijsman, A. Backx, N. G. Kuijpers, M. A. van den Broek, P. Daran-Lapujade, J. T. Pronk, A. J. van Maris, and J.-M. Daran. 2015. CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae. FEMS Yeast Res. 15.

Solis-Escalante, D., M. A. van den Broek, N. G. Kuijpers, J. T. Pronk, E. Boles, J.-M. Daran, and P. Daran-Lapujade. 2015. The genome sequence of the popular hexose-transport-deficient Saccharomyces cerevisiae strain EBY.VW4000 reveals LoxP/Cre-induced translocations and gene loss. FEMS Yeast Res. 15.

Solis-Escalante, D., N. G. Kuijpers, F. H. Linden, J. T. Pronk, J.-M. Daran, and P. Daran-Lapujade. 2014. Efficient simultaneous excision of multiple selectable marker cassettes using I-SceI-induced double-strand DNA breaks in Saccharomyces cerevisiae. FEMS Yeast Res. 14:741-754.

Kuijpers, N. G., S. Chroumpi, T. Vos, D. Solis-Escalante, L. Bosman, J. T. Pronk, J.-M. Daran, and P. Daran-Lapujade. 2013. One-step assembly and targeted integration of multigene constructs assisted by the I-SceI meganuclease in Saccharomyces cerevisiae. FEMS Yeast Res. 13:769-781.

Kuijpers, N. G., D. Solis-Escalante, L. Bosman, M. A. van den Broek, J. T. Pronk, J.-M. Daran, and P. Daran-Lapujade. 2013. A versatile, efficient strategy for assembly of multi-fragment expression vectors in Saccharomyces cerevisiae using 60 bp synthetic recombination sequences. Microb.Cell Fact. 12:47.

Solis-Escalante, D., N. G. Kuijpers, N. Bongaerts, I. Bolat, L. Bosman, J. T. Pronk, J.-M. Daran, and P. Daran-Lapujade. 2013. amdSYM, a new dominant recyclable marker cassette for Saccharomyces cerevisiae. FEMS Yeast Res. 13:126-139.