MicroTime 200 PicoQuant Lifetime detection
Purpose:
- Confocal for time-resolved fluorescence measurements (FCS, FLIM, FRET)
Confocal pinholes available: 30, 50, 75, 100, 150 µm
Microscope stand: Olympus IX81, Inverted
Illumination and detection system: Picoquant MicroTime 200
Fluorescence excitation: Picoquant picosecond pulsed lasers
Laser line (nm) | Max output power @ 40 MHz (mW) | Pulse duration (ps)
|
404 | 3 | 54 |
485 | 5 | 93* |
530 | 2 | 83 |
640 | 4 | 63 |
*2 laser sources with orthogonal polarization
Detection: 2 channels
Detection device: 2x SPAD from Micro Photon Devices (MPD), PDM series
- Single-photon detectors
- Quantum efficiency: 50% @ 550 nm
- Timing accuracy: < 50ps
Control and acquisition software: Picoquant SymPhoTime
Additional features:
- XY mechanical stage
- Objective scanning mode: XYZ piezo stage (±100 µm)
- Z drift compensation module Olympus ZDC2
Objectives (from Olympus):
| Objective type | Mag/NA | Medium | Contrast | WB (mm) |
UPlanSApo | 60x / 1.2 | Water | Fluo, DIC | 0.28 |
Dichroic mirror for confocal illumination:
| Laser lines reflected | Reference |
| 530/640 | Z532/635 rpc |
480/640 | 480/640 FITC/Cy5 |
| 405 | ZT405 rdc |
| 480/530 | Z480/532 rpc |
Fluorescence emission filters:
| Emission filter | Type | Reference |
| 520/35 | Band-pass | FF01-520/35 (x2) |
542/25 | Band-pass | FF01-542/25 |
| 690/70 | Band-pass | HQ690/70 |
| 430LP | Long-pass | HQ430LP |
| 563/9 | Band-pass | HC563/9 |
| 580/70 | Band-pass | HQ580/70 |
Dichroic beam-splitter for dual-channel detection:
| Dichroic filter | Reference |
| 620 | 620DCXXR (x2) |
| 500 | 500DCXR |
| 532 | Z532 RDC |
| 638 | Z638 RDC |
Optional- Correlation with conventional Widefield detection:
Illumination: High-power lamp Andor AMH-200-F6S
Detection: 1 channel
Detection device: EM-CCD Andor Luca R
Chip size: 1004x1002 pixels
Pixel size: 8x8 µm
Dynamic range: 14 bit
Maximum frame rate: 12 frames/s (full chip)
Quantum efficiency: 65% @ 600 nm
Control and acquisition software: Andor IQ3
Widefield epi-fluorescence filter cubes installed in the microscope:
| Excitation filter | Dichroic filter | Emission filter | Filter cube reference |
| 482/18 | 495 | 520/28 | GFP-1828A-OMF-ZERO |
| 443/22 | 562 | 593/40 | TRITC-B-OMF-ZERO |
| 628/40 | 660 | 692/40 | Cy5-4040B-OMF-ZERO |
