Model intercellular bridge with septin and other proteins (MEP)

Short description:
In the intercellular bridge, proteins are important for the recruited and organized in the intercellular bridge. In our group, we use cell-sized giant unilamellar vesicles (GUVs) as a model to investigate biological proteins spatiotemporal interactions in a bottom-up approach. By utilizing microfluidic traps, the geometry of the plasma membrane is altered to investigate the shape deformations. Experimentally you can learn about encapsulation of proteins, such as septin and anillin, to model the intercellular bridge. Additionally, you can improve the quantification of the co-localization of multiple proteins to each other or the membrane.

Techniques/methods applied in the project:
Protein encapsulation, confocal microscopy, image analysis, python coding

Relevant publication for the project:
An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis., TC Panagiotou, A Chen, A Wilde, Cell Rep. 2022; 40(9):111274. doi: 10.1016/j.celrep.2022.111274. PMID: 36044846.

Contact
SaFyre Reese (S.D.R.MxReese@tudelft.nl) and Prof. Dr. Gijsje Koenderink (G.H.Koenderink@tudelft.nl)